Amplification of a cytochrome P450 gene is associated with resistance to neonicotinoid insecticides in the aphid Myzus persicae.
Amplification of a cytochrome P450 gene is associated with resistance to neonicotinoid insecticides in the aphid Myzus persicae.
Blog Article
The aphid Myzus persicae is a globally significant crop pest that has evolved high levels of resistance to almost all classes of insecticide.To date, the neonicotinoids, an economically important class of insecticides that 4 Piece Queen Poster Bed target nicotinic acetylcholine receptors (nAChRs), have remained an effective control measure; however, recent reports of resistance in M.persicae represent a threat to the long-term efficacy of this chemical class.In this study, the mechanisms underlying resistance to the neonicotinoid insecticides were investigated using biological, biochemical, and genomic approaches.Bioassays on a resistant M.
persicae clone (5191A) suggested that P450-mediated detoxification plays a primary role in resistance, although additional mechanism(s) may also contribute.Microarray analysis, using an array populated with probes corresponding to all known detoxification genes in M.persicae, revealed constitutive over-expression (22-fold) of a single P450 gene (CYP6CY3); and quantitative PCR showed that the over-expression is due, at least in part, to gene amplification.This is the first report of a P450 gene amplification event associated with insecticide resistance in an agriculturally important insect pest.The microarray analysis also showed over-expression of several gene sequences that encode cuticular proteins (2-16-fold), and artificial feeding assays and Home in vivo penetration assays using radiolabeled insecticide provided direct evidence of a role for reduced cuticular penetration in neonicotinoid resistance.
Conversely, receptor radioligand binding studies and nucleotide sequencing of nAChR subunit genes suggest that target-site changes are unlikely to contribute to resistance to neonicotinoid insecticides in M.persicae.